Education
•1995-1999
B.S. in Biochemistry, College of Life Sciences, Sichuan University, Chengdu, China
•1999-2004
Ph.D. in Biochemistry and Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Research Projects:
1. Clone, Expression and Characterization of human aminoacyl-tRNA synthetases (tryptophanyl- and tyrosyl-).
2. Mechanistic Study of Species-specific (bacteria and human) recognition of tryptophanyl-tRNA in protein translation.
The findings from these projects were published in the prestigious scientific journals, including Journal of Biochemical Biology, Biochemical Journal, and Protein Expression and Purification.
•2004-2008
Postdoctoral Fellowship in Cell Biology and Immunology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio
Research Projects:
1. Identification, characterization and reconstitution of GAIT complex (a protein translational suppressor) in human myeloid cell inflammation.
2. Investigation of GAIT complex-related regulatory network in inflammatory gene expression.
The findings from these projects were published in the prestigious scientific journals, including Nature, Molecular Cell and Proceedings of National Academy of Sciences.
Working Experiences
•Mar.-Jul. 2004
Assistant Investigator, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Job Summary: Assistant to senior investigator for lab management. I oversaw research project development, provided professional training to graduate students, and led the project of protein expression, purification and characterization.
•Jul.2004-Dec.2008
Postdoctoral Research Fellow, the Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio
Job summary: My major project was to investigate the molecular mechanism of GAIT complex-mediated gene regulation in macrophage inflammation. I played the leading role in experimental design, execution, data analysis and documentation. I cloned, expressed and purified four GAIT protein components in different protein expression and purification systems. And I independently developed several analytical approaches by using Biacore, CD spectrometry, and FRET for the study of protein-protein interaction and protein binding kinetics in GAIT complex assembly. Completion of this project extended my research experience of protein biology to the study of chronic inflammatory diseases.
•Jan.2009-Mar.2013
Research Associate, Department of Cellular and Molecular Medicine; Manager of Biochemical and Biophysical Core, Cleveland Clinic, Cleveland, Ohio
Job summary: After I promoted to Research Associate Staff, I developed an independent research project to study the pathological mechanism of how oxidized low-density lipoprotein causes chronic inflammation in atherosclerosis. In this project, I identified a novel protein complex that has S-nitrosylase activity and regulates protein function via selective S-nitrosylation. I identified and validated complex protein components by immunoassays and LC-MS analysis. Moreover, I developed a few Biacore applications for the determination of protein binding kinetics, for validation of protein-protein interaction, and for high-throughput screening protein substrates and inhibitory peptides. Because of my expertise of protein analysis, I received a joint appointment as Research Core Manager for two NIH funded projects. My major responsibility in this position was to oversee equipments operation (Biacore 3000, iTC and CD spectrometry), supervise lab personnel...
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