RESEARCH EXPERIENCE
Icahn School of Medicine at Mount Sinai, New York, NY
Graduate Student with Dr. Jordi C. Ochando, 2012-present
Functional and mechanistic studies of myeloid-derived suppressor cells and their role in establishing transplantation tolerance
• Characterized myeloid-derived suppressor cell population revealed to contribute to induction of transplant tolerance in mice that underwent cardiac transplants by flow cytometry and functional suppression assays
• Identified biochemical pathway controlling myeloid-derived suppressor cell function in transplantation setting
Icahn School of Medicine at Mount Sinai, New York, NY
Graduate Student with Dr. Thomas M. Moran, 2009-2012
Functional and mechanistic studies of T-cell mediated anti-viral responses during pregnancy
• Developed method for naïve and memory T-cell stimulation, antigen-presenting cell free and coculture from peripheral blood mononuclear cells of donor buffy coat fractions.
• Analyzed potential effects of pregnancy associated steroid hormones on cytokine production by stimulated T-cells by sandwich ELISA. Extracellular and intracellular flow cytometry for secreted and non-secreted protein production.
• Analyzed potential effects on transcription by qRT-PCR
Stony Brook University, Stony Brook, NY
Research Assistant with Dr. Dale G. Deutsch, 2005-2008
Biochemical studies of hippocampal anandamide synthesis
• Developed transgenic mouse line for conditional knockout of NAPE-PLD
• Developed thin-layer chromatography assay for NAPE-PLD activity
SKILLS and TECHNIQUES
• Isolation of RNA and analysis by transcriptional profiling on microarrays and qRT-PCR
• Statistical analysis of microarray data
• Immunohistochemistry and fluorescent microscopy
• Extracellular and intracellular protein analysis by fluorophore staining, examination, and sorting using flow cytometry (instruments: LSRII, FC500)
• Analysis of flow cytometry data
• Sterile mammalian cell culture
• Isolation of DNA and genotyping analysis by PCR
• Prokaryotic and Eukaryotic cloning by electroporation
• Fusion protein design and integration
• Transgenic mouse colony development and maintenance
EDUCATION
Icahn School of Medicine at Mount Sinai, Graduate School of Biomedical Sciences, New York, NY
• Ph.M. in Biomedical Sciences 2013
State University of New York at Stony Brook, Stony Brook, NY
• B.E., Biomedical Engineering 2008
• Suma cum Laude
• Tau Beta Pi Honor Society
• Golden Key International Honor Society
Suffolk County Community College, Selden, NY
• A.S., Biology 2004
GRANTS and AWARDS
Undergraduate Research & Creative Activities (URECA) Fellowship 2007
Howard Hughes Medical Institute Fellowship 2006
Computer Science, Engineering, and Mathem...
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