Biomedical Engineer
TECHNICAL SKILLS AND RESEARCH TECHNIQUES
Bioengineering: Chemical micropatterning, stem cell patterning, mammalian cell and tissue culture, chemical surface modification, self-assembled monolayers, microfabrication,cellular microfluidics, biomaterials, in vitro experimentation, point-of-care detection devices,biocompatibility.
Micro/Nano Technologies: Fluorescence/Confocal Microscopy, UV-spectrophotometery,SEM, AFM, 3D Non-Contact Profilometer, Soft-photolithography, Spin Coating, Sputter Coating, Laser Machining.
Experimental Techniques: Immunofluorescence Assay (IFA), PCR, SDS-PAGE, cell
culture and tissue culture, chemical surface modification, cellular microfluidics, hydrogel,biomaterials.
Computer Skills: Microsoft Office Suite, MATLAB, AutoCAD, LabVIEW, ImageJ, InStat3,Minitab, SigmaPlot, CorelDRAW, Linux, C, UNIX.
Validation: Process and software validation, PFD, PFMEA, ERS, FRS, IQ, OQ, PQ, SOP,Traceability Matrix.
Government Regulations: 21 CFR Part 11, 210, 211, 820, cGMP, cGLP.
EDUCATION
Dec 2011 MS in Engineering Mechanics (Focus: Biomedical Engineering)
University of Nebraska, Lincoln, NE
May 2009 BS in Biological Systems Engineering, Minor in Biomedical Engineering
University of Nebraska, Lincoln, NE
WORK EXPERIENCE
Research Tech
Mar 2013-Present
Harvard-MIT Health Sciences and Technology (HST), Harvard Medical School
Brigham and Women’s Hospital, Cambridge, MA
Design and fabricate PMMA/PDMS based Micro-chip for cellular and point of care
microfluidics devices.
Application of microfluidic chip-based 3D co-culture to regenerate functional bone tissue model, and micro-vascular network.
Design and apply microfluidic chip based cell culture to test drug sensitivity forindividualized treatment of human breast cancer.
Create and analyze functional 3-D in vitro Neuronal Circuits using human iPS cells, and mouse embryonic stem cells.
Perform SPR and WGM for bacterial detection using microfluidic chips.
Synthesize GelMA hydrogel.
Perform immunocytochemistry and fluorescence/confocal microscopy.
Engineer Sep 2012-Mar 2013
Terumo Cardiovascular Systems, Ann Arbor, MI
Via EmrIT Solutions Inc.
Demonstrated primary commitment to product quality by maintaining compliance to the Quality Policy and all other documented quality processes and procedure.
Provided technical assistance to plan and implement various projects including planning and scheduling, coordinating resources, and preparing documentation.
Assisted with cause-identification and problem-resolution for various types of production or service-related issues.
Provided engineering input in desnd PFMEAs.
Developed ERS, IQ, OQ and PQ for test fixtures, equipment, and executed the protocol.
Provided assistance for planning and implementation of projects to develop, install, and qualify new processes for manufacturing new products or improving existing processes.
Supervised and reviewed QA effort and facilitated the implementation to meet 21 CFR Part 11 requirements
Responsible for ensuring the compliance of GMP/GLP and FDA regulations.
Developed Standard Operating Procedures (SOPs) in compliance with FDA regulations.
Analyzed System Requirements to document in Functional Requirements Specification
FRS) and Design Specifications (DS).
Prepared Traceability Matrix to define the relationship between requirements, design specifications and test scripts.
Developed Test Summary Report to summarize all test activities done in controlled test environment.
Research Tech
Jan 2012-Jul 2012
University of Nebraska, Lincoln, NE
Department of Mechanical and Materials Engineering
Designed, executed and scrutinized mechanotransduction study on stem cells by using isolated and interconnected enzyme microarrays, and through gene silencing.
Performed microfabrication to create microfluidic channels on 1-4” silicon wafers through the use of positive and negative photoresists for cancer cell migration, movement and
spatial localization.
Developed Neuronal Network Formation Assay (NFA) using human neuronal precursor(SH-SY5Y) cells.
Assessed effect of interconnectivity on stem cells (C3H10T½) in cadherin expression and osteogenesis using protein microarrays.
Supervised graduate/undergraduate students in performing their daily laboratory tasks and assisting them in understanding laboratory techniques and equipment.
orked closely with principal investigator in writing grant proposal manuscripts, new funding opportunities and research projects.
Maintained laboratory supplies, ordering and inventory.
Troubleshoot laboratory equipment on a regular basis.
Graduate Research Assistant
Jul 2009-Dec 2011
University of Nebraska, Lincoln, NE
Department of Engineering Mechanics
Established soft-photolithography and microcontact printing facility, and devolved standard operating procedures.
Developed photomask with various micropatterns to perform neuronal and osteoblastic differentiation of adult stem cells.
Generated protein microarrays of collagen, laminin, fibronectin and lysine by using PDMS microfluidic devices.
Performed microcontact printing of extra cellular matrix proteins and chemicals on glass slides, tissue culture plates and PDMS surfaces in making microarrays ranging from 2-75 µm in size.
Accomplished chemical surface modification of PDMS, glass and polystyrene, and
evaluated the effect of shape and size of ECM protein microarrays on stem cell behavior and their fate.
Appraised neuronal and osteogenic differentiation of stem cells by performing biomolecular assays.
Conducted thorough assessment to further understand mechanism of Traumatic Brain Inju...
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